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Cooperative effects throughout the peptide binding site would also provide an explanation for the fact that both types of binding energy available to the peptide/MHCII complex (hydrophobic interactions and hydrogen bonding) can influence DM stability [16], [24].
Both types of binding were effective in improving osteoblast function.
Furthermore, FAIRE-seq analysis demonstrates that both types of binding region exhibit increased chromatin opening, which also occurs more rapidly in the Otx2+/Oct4+ group.
Many of these promoters actually contain both types of binding sites, and in opposing-strand orientation, potentially indicative of combinatorial regulation (Additional file 5 Figure S5).
Using single molecule force spectroscopy, we have demonstrated that the full-length Pol III α subunit binds to both dsDNA and ssDNA and have quantified the affinity for both types of binding.
First, the genes containing transcriptional factor A binding sites (TFA) and transcriptional factor B binding sites (TFB) in the promoter regions can be denoted by TFA ∩ TFB, which is a subset of genes containing both types of binding sites.
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Scatchard plot analysis of binding curves from both types of equilibrium binding assays revealed a Hill coefficient >1, suggesting cooperativity of ABA binding to LANCL2.
It is possible that, if strain 50/97 produced a CRASP-1 or CRASP-2 homolog, the ospE transformant would express both types of CFH-binding proteins on its surface, and thereby bind CFH at levels sufficient to prevent complement-mediated killing.
For both types of resin, the binding capacity decreased slowly with increasing NaOH concentration.
Similarly, HB-19/Btn binds and forms a stable complex with surface nucleolin in a dose-dependent manner in both types of cells, with maximum binding occurring at 8 12 µM concentration (Figures 2A, 3A).
DNA-binding specificities of TFs are encoded in their DNA-binding domains that specialize them to recognize and bind specific types of binding sites.
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