Xenograft models for lung cancer have advantages and disadvantages in comparison with classic transgenic and conditional murine models.
Finally, verification of newly discovered genes is possible in genetically engineered mice (e.g. transgenic and conditional knockout mice).
Those transgenic and conditional knock-out models relevant to lung injury, inflammation and development will be discussed in more detail in the sections below.
However, transgenic and conditional transgenic model systems that accurately mimic tumour histology, genetic abnormalities and tumour microenvironment of human lung cancer, such as the LSL K-Ras G12D model for adenocarcinoma, and the Trp53 AdenoCre model for SCLC, may provide more reliable results concerning response to therapy and toxicity.
Mice exhibit a morphologically analogous form of small intestinal villus epithelial cell shedding to that observed in humans and have the potential to allow important mechanistic insights because of the availability of established transgenic and conditional mutant models.
In this review we present what is known about Myc from transgenics and conditional knockout models, and also include indirect evidence implicating Myc based on results from other studies.
Therefore, in combination with transgenics and conditional knockouts, ENU mutagenesis will continue to play an important role in the identification and characterization of mutations that underlie human neurological disease.
Lentivirus infection has been used effectively to generate stable transgenic mammalian lines with both constitutive and conditional expression of transgenes and shRNAs [8], [9], [10], [31], [32].
(B ) Western blot showing endogenous and conditional transgenic Akt1 expression in response to Tam and LY treatment.
In this chapter, the types of genetically engineered mice, such as transgenic, knockout conditional knockout, and knockin animals, and the general techniques on how to obtain them are described.
