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Both transcripts were detected in normal prostate cells, as well as prostate tumor cells, suggesting that ALT-PTK6 is not restricted to cancer cells (Figure 1C).
As shown in Fig. 4A, both transcripts were detected in skin, thymus, stomach, tonsils, testis and placenta, but not in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal gland (data not shown).
Both transcripts were detected in wild-type Columbia (Col) buds (Fig. 2).
Among normal tissues, placenta expressed almost only DCD-SV, whereas in normal breast both transcripts were detected at a 2 1 ratio and cell lines displayed variable DCD and DCD-SV expression levels (data not shown).
After 6 to 8 h of exposure to salinity, increasing levels of both transcripts were detected in young leaves, suggesting preferential CK biosynthesis in photosynthetic organs and thus underlining CKs stress-prevention and anti-senescence properties.
It was shown that at the time of sampling, TRV1 but not TRV2 transcripts were detected, although both transcripts were detected a few weeks earlier in the same plant.
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Both GS2 transcripts were detected only at basal levels in mature dry seeds and in seeds 1.5 hours following imbibition.
In the leaves both isoform transcripts were detected, but while the NAD(P H-GOGAT did not show any significant differences, the Fd-GOGAT showed a decrease in its exP H-GOGATwith time.
As expected of a heterozygous individual, both normal and mutant transcripts were detected.
Both alternative 3′ exon transcripts were detected in all tissues analyzed, with the levels being highest in the lung, thymus, and spleen.
In both cases host actin transcripts were detected, yet primer pairs specific to the parasite genes yielded signal consistent with background.
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