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Dysbindin depletion up-regulated both total and surface-localized FLAG-DOR levels at steady state in HEK293 cells (Figs 3A and B).
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We used immunofluorescence staining to visualize total and surface-localized endogenous TfR (i.e. with and without cell permeabilization) (Fig. 3B).
Both total and surface protein samples were boiled for 5 min at 95°C.
Both total and surface CaSR expression, that is fluorescence intensity, are presented as a percentage.
Together with our previous data excluding parasite microneme- and surface-localized factors from stimulating EGR expression [24], these data indicate that SRF activation is mediated by a parasite-derived rhoptry- or dense granule-localized factor.
Samples of both total and cell-surface protein were analysed by Western blotting as above.
Both total and ferrous iron are depleted from the land surface of catena soils relative to the bedrock.
Treatment with extensin inhibitor 3,4-DHP depleted surface-localized JIM11 and JIM20 epitopes and also disrupted ECM.
These results suggested that oligomerization of p62 results in clustering of the ubiquitin-binding UBA domain and thus avid binding to surface-localized, clustered ubiquitin, analogous to the interaction with surface-localized LC3B.
In contrast to Gpr177, the amount of surface-localized Fzd receptors and Lrp6 co-receptor did not change (Fig. 2F).
Evolutionary tracing analysis and structural modeling identified a cluster of surface-localized amino acids fully conserved between the Ig domains of all known β3 and β1 sequences.
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