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14 miRNAs were deregulated in both tissues and cell lines and are listed in Figure 3A.
miR-221 was the most highly elevated miRNA in both tissues and cell lines (9-fold and 7-fold respectively), while miR-21 was significantly decreased in both sample types (3-fold and 9-fold, respectively) (Table 2).
miR-424 and let-7f were downregulated in both tissues and cell lines (Figure 3A), while miR-34c and miR-98 were found downregulated in ovarian cancer tissues and in cell lines, respectively.
Analyses of the glycoproteins residing at the cell surface, in both tissues and cell lines, enabled discovery of cell-membrane markers that may be shed and released into the peripheral circulation.
The comparison was made between assay N°3 (selected new assay) and the original assay N°1 (reference assay) considering samples derived from both tissues and cell cultures from different species of salmonids.
While some differences clearly exist, the most abundant protein classes (cell adhesion, defense/immunity, enzyme modulator, extracellular matrix, hydrolase, protease, receptor, signaling, transfer/carrier and transporter) were resolved in both tissues and cell lines.
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Both tissues and cells were washed with PBS and incubated with CMFDA for 30 min. Next, the unbound CMFDA was washed of by washing with PBS for 2 times.
Thus a number of TFs, in particular AP2 and IRX family members and SOX15 have a similar expression pattern to p63 (ΔNp63) across both tissues and cell-lines.
The expression of steady-state ABHD1 and Sec12 mRNA in both tissues and cells showed that these two genes are co-expressed in a spatial manner.
Expression of CYP3A genes is both tissue and cell specific and exhibits inter-individual variation [11].
Nuclear protein fractions from both tissue and cell samples were used for TransAM assay.
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