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Both tibias were equal in length initially.
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Acceleration of C7, T7, T12, both femurs, and both tibias were also measured for the PMHSs.
One week after surgery, the animals were killed with carbon dioxide, and both tibias were harvested for evaluation.
At the end of the study animals were euthanized and both tibias were extracted for ex vivo high-resolution 3-D micro-computed tomography (μCT) imaging.
Ten-week-old mice were euthanized in a CO2 chamber; both tibias were removed, dissected free of surrounding soft tissue, weighed and immediately frozen.
The length of both tibias was measured from the AP views.
Consequently, for each animal, the bone marrow of both tibias was flushed with 200 μl of DMEM and mixed before analysis for Dkk-1 levels.
Both teams were equals.
Briefly, both femurs and tibias were collected after mice were sacrificed.
Both femora and tibias were isolated and rinsed with phosphate-buffered saline (PBS; Gibco, USA).
Both femurs and tibias were fixed in 4% paraformaldehyde at pH 7.4 for 3 days, were decalcified in 20% ethylenediamine tetraacetic acid solution for 21 days, and were then embedded in paraffin wax.
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