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Further, in several generations, both tibia length and body mass decreased in the Longshanks lines.
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Individuals were first ranked by absolute tibia length, and then again by relative tibial length, i.e., tibia length divided by (body mass)^0.33.
Tibia length and distance between the malleoli and the Achilles tendon were measured.
After statistical analysis, the most sensitive responses for detecting differences between formulations were body weight, tibia length and organ weights.
Second, we determine to what degree the genetic covariance between tibia length and body mass has potentially and actually constrained the evolution of both traits within the experiment.
Total changes in tibia length and body mass were obtained by summing across generations.
Theoretical changes in body mass, tibia length and relative tibia length over future generations of selection in Longshanks Line 1, assuming similar selection regimes and genetic variance/covariance structure.
Through the existence of a positive genetic correlation, selection against increases in body mass has reduced the actual selective gains in tibia length, and by the same token the rate of evolutionary change in tibia length.
In all models, the regression coefficients for both stature and tibia length were highly significant and similar in magnitude (B = 0.61 0.65, R = 0.18 0.23).
Additionally, we measured snout-vent length (SVL) and body mass of the metamorphs and juveniles and in adults also the tibia length (TL) and the callus length (CL).
We measured the following morphological traits: carapace width (CW), right foreleg tibia length (FTL) and foreleg tibia diameter (FTD).
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