Brush a homemade or commercial sauce, like a barbecue sauce, over the chicken thighs using a basting brush.
One hour after 18F-FLT injection, CT commenced from the vertex or orbitomeatal line and progressed down toward the upper thigh using a standard protocol, 120 kVp, 100 mA, and 4-mm slice thickness.
Anesthesia was delivered in the caudal thigh using a 23 gauge sterile syringe needle.
The resulting emulsion was injected subcutaneously into the lateral thigh using a glass syringe.
Liraglutide (active or placebo) was injected subcutaneously once daily at any time of the day in the upper arm, abdomen, or thigh using a prefilled pen device.
Liraglutide (active or placebo) was injected subcutaneously once daily at any time of the day in the upper arm, abdomen, or thigh using a pen injector device.
Proximal thigh muscle bulk was evaluated by measuring the circumference of the thigh using a measuring tape with a spring-loaded marker (ReDog) to ensure that tape tension was identical for each measurement.
Percutaneous needle biopsies were obtained from the vastus lateralis, ∼12 13 cm above the patella on the anterolateral aspect of the right thigh using a Bergstrom needle (Stille, Solna, Sweden) as previously described (28).
PET scan was then performed from the head to mid-thigh using a PET scanner (ECAT EXACT HR+, Siemens, Erlangen, Germany) or PET/CT scanner (Biograph Duo or 40, Siemens, Erlangen, Germany).
After 1 h, the patients were scanned from the head to mid-thigh using an integrated PET-CT system.
