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This interpretation is further supported by the observed differences in both the sedimentation coefficient and the electrophoretic mobility of the 1 1 complex formed on the RND substrate.
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The biggest difference is seen in the reduction of the sedimentation coefficient, which is caused by both an increase in ν̅, and an increase in the frictional ratio.
The sedimentation coefficient distributions were obtained using the c(s) method of SEDFIT ver. 15.01b61.
Using this model the sedimentation coefficient of an individual macromolecule is recalculated into the sedimentation coefficient of semidilute solution.
Data analysis was carried out using SEDFIT40, which employs the continuous c(s) conformational change model based on the Lamm equation, to determine the sedimentation coefficient distribution.
Radial scans at 280 nm were taken every 10 min and the sedimentation coefficient distribution was calculated by least squares boundary modeling of the sedimentation velocity data using the programme SEDFIT.
The native trimeric enzyme has a sedimentation coefficient of 5.95 S. Removal of the weakly associated metal results in no change in the sedimentation coefficient.
The sedimentation coefficient distribution, c(s), was calculated at a confidence level of p = 0.68.
The sedimentation coefficient distribution c(s) was calculated with maximum entropy regularization at a confidence level of p = 0.7 and at a resolution of sedimentation coefficients of n = 100.
Although the sedimentation coefficient distribution plots showed peaks at intermediate positions between the monomer and pentamer peaks (Figs.
Apparent sedimentation coefficients were determined by the sedimentation coefficient distribution C(S) generated by SEDFIT program [ 18].
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