Given that there were significant differences in copulation duration of the sterile F1QW male hybrids based on whether they mated to D. w. willistoni or D. w. quechua females (F1,58 = 4.27; P = 0.004) (Additional file 1: Table S1), we re-analyzed the data by partitioning the mating types by both females and males involved.
(D ) The importance of repressing haploid-specific gene expression for the evolved phenotype was assessed by comparing cells that expressed both alleles of the mating type information (HMLα ) to cells that expressed only MAT a information (hmlαΔ ).
The mating types of 124 and 88 single spore isolates, isolated at random from Volvariella volvacea strains V23 (A mating type locus alleles A1 + A2) and PY (A mating type locus alleles A3 + A4) respectively, were determined by molecular screening using four primer pairs designed according to the sequences of the A mating type genes (HD1 and HD2) of the two parental strains.
In both species, recombination is abolished around the mating type locus, creating a large haplotype that may accumulate genetic polymorphisms, but the solutions found by the 2 fungi to inhibit it are quite different.
Amplification and sequence analysis of the sex specific SXI1α mating type gene, as well as the absence of the mating type specific SXI2a gene, confirmed that both isolates had the α mating type allele (MATα).
[ T + N] and [ Dp + Df] mycelia were distinguishable because the former yielded self-sterile homokaryotic conidial derivatives of both mating types, whereas the latter produced homokaryons of only the mating type of the Dp component.
The [ T + N] and [ Dp + Df] heterokaryons are distinguishable, because the former produces homokaryotic conidial derivatives of both mating types, whereas the latter produces viable homokaryons of only the mating type of the Dp nucleus.
[ T + N] heterokaryons produced homokaryotic conidial derivatives of both mating types, but [ Dp + Df] heterokaryons produced viable conidial homokaryons of only the mating type of the Dp nucleus.
A likelihood-based approach provides a valid substitute to TDT-based method by performing stratification on both the parental mating type and the inherited number of copies of the allele under study [ 66], allowing for reliable testing of both imprinting and maternal effects.
Primers were subsequently designed to target the MAT1-1-1, MAthe1-3 (of the mating type 1 idiomorph), and MAT1-2-1 (of the mating type 2 idiomorph) genes in these isolates.
