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In specific instances during the secretory stage, the ameloblasts separated from both the dentin surface and the stratum intermedium, but remained loosely attached to each other.
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Both mechanisms seem to prevent optimal adaptation of the adhesive into the dentin surface, and consequently bond strength and marginal integrity is compromised [15].
The smear layer is composed of small particles of dentin and dendrites covering the dentin surface.
The excess water was blot dried with absorbent paper, leaving the dentin surface visibly moist (wet bonding).
These scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used.
For hypersensitivity to occur, the exposed dentin must have at least two hyperconductive characteristics, open tubules on the dentin surface and that lead to vital pulp [3],[4].
Five silicon molds of 1 mm high and 1 mm of internal diameter were distributed through the dentin surface already prepared with the adhesive techniques described above.
Excess water was removed by air-drying for 5 s and absorbent paper (Kleenex Classic, Kimberly-Clark, Suzano, SP, Brazil), leaving the dentin surface visibly moist.
Then, the matrix was gently removed, resulting in specimens with three cylindrical restorations at the dentin surface, which were stored in distilled water at 37°C.
In this case, chemical bonding occurs by the formation of hydroxycarbonate apatite on the dentin surface, resulting in a longer lasting interaction with dental tissue [10].
This conditions the dentin surface and allows for contact differentiation of pulp stem cells into odontoblastlike cells, protects dentin from resorption, and enhances cell growth as well as attachment to dentin.
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