Though the budget was originally agreed at £5,000, the final cost of building was £30,000, one third of which was spent on the crypt structure.
Histological analysis of intestinal epithelium in FHL2-null mice revealed no major alterations in crypt-villus architecture of the small intestine and in the crypt structure of the colon (Fig. 1A).
The crypt structure forms an enclosure promoting the nonrandom association of its members.
As such, a single LGR5+ve cell can generate a "mini-gut -like organoid culture in vitro, recapitulating the crypt structure.
In the adult mouse, a proliferative role for this pathway is recapitulated when the Wnt inhibitor Dickkopf-1 is over-expressed, leading to collapse of the crypt structure[ 2], and most notably in disease, where mutations in this pathway result in epithelial hyperproliferation leading to colorectal cancer[ 5].
A test pattern of micro-well features with similar dimensions (50, 100, and 500 μm diameter, 50 μm spacing, 120 μm in depth) to the crypt structures found in native basal lamina was produced in the surface of a poly(dimethylsiloxane) (PDMS) substrate.
The en face view at a depth of 250 μm (Fig. 7 (d)) shows the crypt structures in the rabbit colon.
In the colorectum, CFM detected architectural differences between the normal crypt structure and neoplasia [ 11, 12].
We found substantial histological disruption of the villus and crypt structure concomitant with extensive cellular infiltration in the colonic lamina propria of mice that have been administered with TNBS alone (Fig. 5B) or with non-treated DCs (Fig. 5C).
Despite the reduced cellularity of crypts in these mice, individual epithelial cells appeared to undergo a process of hypertrophy, thus accounting for the maintenance of overall crypt structure observed in vABKO and vTKO mice.
