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As expected, the K203A substitution in CMTr1 strongly affects both the binding and activity of the enzyme, whereas R228A does not (Fig. 5), demonstrating that the method is able to discriminate between the residues that interact with substrate and those that do not.
Susceptibility to PEG-related hindrance is supported by the fact that PEGylation on the hinge region (C194) inhibited activity, in spite of the fact that this region is independent of both the binding and active domains.
TBR1 is involved in both the binding and activation of the Auts2 gene.
Thus, it appears that the residue corresponding to Phe288 affects both the binding and hydrolysis of substrate.
We found that both the binding and signaling data were almost identical to data generated under serum-free conditions (Figure 3 and data not shown).
We also found that both the binding and the unbinding of the quinols is two to three times faster at the Qi site than at the Qo site.
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Above 100 mM K+, only specific binding is observed, and both the binding constant and the magnitudes of enthalpy, entropy and heat capacity changes all decrease strongly with increasing [K+].
The Gerbera proteins were translationally fused separately to both the binding domain and the activation domain, and were combined in both directions.
Two series of random mutagenesis and FACS-based selections (named I and II) were applied to improve both the binding affinity and crossreactivity of three promiscuous clones: CK1, CK2, and CK4.
Both the binding energies and the atomic ratio of boron and nitrogen atoms are close to that of h-BN, implying that the as-prepared products are BNNTs.
Like a number of other briefs in all three cases, the Parliament members' brief argues that the administration's actions violate both the binding obligations and the norms of international law.
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