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Both tests used a validated computerized myometer system (QMA Systems; Gainesville, GA, USA).
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Both tests used an adjusted number of degrees of freedom to compensate for a weak inter-dependence of vector coordinates.
Ours is among the first studies to conduct both TST and QFT-GIT tests using a large sample size which illustrates the realistic implementation of QFT-GIT in a setting with a high burden of TB and HIV.
Both the quantitative and qualitative tests use a dual probe approach where two fluorescently labeled hydrolysis probes simultaneously detect amplicon, providing broader detection and quantification of rare genotype 4 sequences [ 5, 6].
Tests use a small scoop of brain.
Saturday's test used a single decoy.
Both programs were tested using a 11GB BAM input file.
Total nucleic acid was extracted from blood samples from both studies and tested using a S. pneumoniae-specific PCR assay targeting the pneumolysin gene [ 16].
The four samples, not providing the same result for both methods, were tested using a previously published TaqMan based assay [ 10] and by fast COLD-PCR followed by sequencing.
An important difference is that this study assumed an equal test delay of 1 day for both tests, and used a sensitivity and specificity for the chromogenic media-based test of 98.0% and 99.8%, respectively, where we have used 76.6% and 98.6%, based upon a recently performed meta-analysis [28].
Difference in the proportion of futile laparotomies in both arms will be tested using a Chi-square test with a two-sided significance level of 0.05.
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CEO of Professional Science Editing for Scientists @ prosciediting.com