Sentence examples for both tester and driver from inspiring English sources

The Rsa I-digested tester cDNA was ligated with adapters and hybridized with an excess amount of Rsa I-digested driver cDNA to subtract the cDNA population present in both tester and driver.

Both, tester and driver, cDNA populations were processed following manufacturer instructions, with some modifications.

Both tester and driver representations were digested with Sau3AI to remove the R-Bgl adaptors.

It thus appears that very abundant tester-specific transcripts escape normalisation, whereas very abundant transcripts expressed in both tester and driver escape both subtraction and normalisation.

Very abundant transcripts common to both tester and driver may escape both normalisation and subtraction, giving rise to abundant background clones in these libraries.

SSH selection reduces the cloning of abundantly expressed housekeeping genes or genes commonly expressed in both "tester" and "driver" samples, and therefore normalizes the expressed cDNA profiles during library construction.

For further confirmation of subtraction analysis efficiency, expression of the actin gene was examined in both the driver and control samples via 23 and 33 cycles of amplification, respectively, indicating that cDNA homologue was removed from both the tester and driver samples by subtraction.

It is important to note, however, that these results are expected given that SSH methods using PCR-Select cDNA Subtraction (Clontech) have a false positive rate that is caused by the presence of remnant cDNAs common to both tester ("reinfected) and driver ("aposymbiotic) samples.

For the generation of the differentially up- and down-regulated products, the tester and driver cDNAs of both conditions were mixed separately; the hybridization occurred at 67°C for 18 h and the amplification occurred by PCR using the oligonucleotide matching the 24-mer adaptor [ 41].

Tester and driver RNAs were subtracted in both directions to construct two SSH libraries enriched for differentially expressed cDNAs in subolesin dsRNA-injected (reverse-subtracted) and saline-injected (forward-subtracted) ticks.

The SSH was conducted both forwards using RNA prepared from hepatoma and non-hepatoma liver tissues as tester and driver, respectively, to yield the hepatoma (T-N) subtracted amplicons, and reversely using the mRNA prepared from non-hepatoma and hepatoma liver tissues as tester and driver respectively to yield the non-hepatoma (N-T) subtracted amplicons as well.