Other discriminative information includes the mass accuracy, presence of missed cleavage sites, number of peptide termini consistent with the enzymatic cleavage, and etc.
Both SaSTT3A and SaSTT3B were analyzed owning lumenally orienting C terminus, consistent with the previously described (Kim et al. 2005).
Soluble ACE from CHO cells transfected with human somatic ACE cDNA and porcine somatic ACE have C-termini consistent with cleavage of the Arg1203-Ser1204 peptide bond in a stalk region near the transmembrane domain [17].
In contrast, equivalent fusions of the human δOR used as controls, gave opposite results (Figure 4C) indicating an extracellular N-terminus, consistent with the known structure of a member of the GPCR superfamily.
Cross-linked peptides (at α N-terminus to α N-terminus, α N-terminus to β N-terminus) consistent with two of the three structures proposed from homology modeling were detected (Supplementary Figure S-13), validating these structures.
The peptide with an N-terminal threonine residue was less α-helical than the peptide with Ser or Ala at the N-terminus, consistent with previous data on α-helix propensities in general and at the N-terminus in particular.
In the present work, six 60S acidic ribosomal proteins including P0-, P2-, P3-types P3-types were phosphorylated close to their conserved C terminus, consistent with results reported elsewhere [ 45].
Our experiments show, however, that Sirt1 is a monomer that migrates fast in SEC due to an extended conformation, apparently mainly due to a disordered N-terminus, consistent with bioinformatics analyses reported here and previously [ 55].
HE4 is a small (23 27 kDa), secretory protein with hydrophobic amino acids at the N-terminus consistent with a signal peptide which cleaves to yield a mature secretory polypeptide with a consensus site for N-linked glycosylation at amino acid position 15.
The solubility of BAX is effectively regulated by the reduced exposure of its hydrophobic C terminus consistent with its predominantly cytosolic distribution in non-apoptotic cells, whereas only a minor fraction of BAX has been shown to be loosely attached to MOMs.
