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Applicability of the assay was evaluated by tracking protein expression of two GST-fused allergens of biopharmaceutical value in E. coli, GST-Der p 2 and GST-Mus a 5, both targeted to inclusion bodies.
As shown in Fig. 4 wt type prestin and prestin with mutations at Y520 and Y667 were both targeted to the cell surface.
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The truncation analysis of ETR1 indicates that the sequences required for both targeting to the secretory system and retention at the ER are contained within the N-terminal 147 amino acids of ETR1.
A similar protein occurs in A. adeninivorans LS3 and is analogous to Pte1 from S. cerevisiae (Maeda et al. 2006) but both are targeted to peroxisomes.
Although they are both mainly targeted to the membrane, they only partially colocalise (Author response image 1 A and B).
Both enzymes were targeted to chloroplasts and shown to be active in planta.
Both the siRNAs targeted to MBP-1 efficiently knockdown MBP-1 expression.
Both modalities were targeted to hemodynamically unstable patients.
We demonstrated that both channels are targeted to vacuolar membranes.
First, in contrast to many other mutation collaboration models, both mutations are targeted to the physiologically relevant endogenous genetic loci.
Both are predominantly targeted to mitochondria, where they induce clustering and loss of membrane potential, indicative of severe mitochondrial dysfunction.
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