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The PCR products of both systems were sequenced.
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Third, the qPCR systems were tested for specific amplifications from soil DNA extracts and 48 amplicons from each primer system were sequenced.
Clones identified by nonradioactive southern blotting based on digoxigenin-labeling system were sequenced.
The PCR products were visualised on EtBr or SYBR_SAFE-stained gels and 24 randomly chosen cloned ITS fragments from each root system were sequenced, and visualised on an ABI 3730 DNA analyser (Applied Biosystems, Foster City).
The two scoring systems are sequence similarity versus base pair probabilities.
CRISPR (clustered regularly interspaced palindromic repeats -Cas (Crepeats -Casated) systems are sequenCRISPR-associatedive defensystemsinst phares and plasmids which are widespread in prokaryotes.
These systems are sequence-specific restriction enzymes, also called restriction endonucleases that have been known for a long time to act as a protection from foreign DNA, such as bacteriophages [ 62].
PCR products were sequenced directly or were cloned into the pGEM-Teasy vector system (Promega) and both strands were sequenced by a commercial sequencing service (StarSeq).
The amplified products were cloned into the pGEM-T easy vector system (Promega) and six clones were sequenced in both the forward and reverse directions for each gene.
Amplified fragments were cloned using pGEM-T Easy Vector system (Promega) and 10 20 clones were sequenced.
The genome of both, the parent strain and the mutant derived from it, were sequenced using the Roche/454 system.
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