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In contrast, we found that both surface and total levels of ErbB2 are actually little changed in the absence of POH1 activity.
A multivariate linear regression model was used for assessment of independent variables correlated with both surface and total CaSR expression.
Indeed, no association was found between age, gender or clinical parameters with both surface and total CaSR expression.
Exposure to CO resulted in a significant increase in expression of both surface and total MCSFR expression while lack of endogenous CO production in myeloid cells from Lyz-Cre:Hmox1 flfl mice resulted in attenuation of MCSFR expression.
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While neither the surface nor the total O/Ti ratio varied significantly with VB and the OTP ratio, both the surface and total C/Ti ratios varied strongly with these parameters.
To measure both cell surface and total expression of CLR an ELISA was carried out as described previously [5], but using the antibodies and o-phenylenediamine tablets described in the Materials section.
After 30 min UV light stimulation (0.3 sec of blue light followed by 1 sec of UV light, repeated every 20 sec for 30 min), both the surface and the total amount of AMPARs are significantly decreased at the activated synapses compared with surrounding normal synapses.
Surface and total expression were measured by adding α-flag FITC with and without Triton, respectively.
Five were used for surface and total porosity measurement and five for elution testing.
Mitochondrial surface and total cell areas were calculated using the same software.
Second, we compared surface and total protein expression of FcεRI-α chain using flow cytometry and immunofluorescence, respectively.
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