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Accordingly, we examined both surface and intracellular BST-2 induction by flow cytometry together with mRNA expression levels and, collectively, our data show that IL-27 induces lower BST-2 expression on T cells compared to that induced by type I IFN.
We have analyzed thymocytes for both surface and intracellular alpha-chain expression and find that the majority of mature thymocytes express a second alpha-chain intracellularly.
Lymphocyte subsets were analyzed for detection of both surface and intracellular proteins as described previously [3].
Cell permeabilization with the use of methanol had a significant influence on the quality of both surface and intracellular staining.
Recent advances in flow cytometry have expanded the number of simultaneous analyses to greater than 13, allowing for the detection of both surface and intracellular factors, thereby enabling identification of specific cell subsets as well as their functional activation in a heterogeneous cell population.
The CD204-FITC antibody was used for both surface and intracellular staining.
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Active Src co-localised with ROR2-myc WT in both cell surface and intracellular locations indicative of a role in intracellular trafficking (Fig. 3A).
So, both cell surface and intracellular molecules play pivotal roles in cancer pathobiology [ 35].
Immunofluorescence in permeabilized cells allows detecting both the surface and intracellular immunoreactivities.
RNS production induces nitration of both membrane surface and intracellular proteins, which mainly results in post-translational modifications of tyrosine residues (96).
On the other hand, in the co-immunoprecipitations, all HA-tagged hIPs (both cell surface and intracellular) were immunoprecipitated and hence, as is suggested from the co-immunoprecipitations, Rab5 associates with the hIP even in the absence of agonist.
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