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The moisture contents of both substrates were determined after drying the samples in an oven at 60 °C for 48 h.
Next, the Raman spectra of PATP immobilized on both substrates were determined at various spots.
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Frequencies of translocation formation in diploid strains subsequent to an HO endonuclease-mediated DSB adjacent to the his3-Δ5' substrate, or both the his3-Δ5' and his3-Δ3' substrates were determined as described previously [38].
These substrates were determined both in arterial (radial artery) and in venous blood of the unaffected arm.
The kinetic constants for the three substrates were determined for both enzymes using a coupled enzyme assay.
The mechanical properties of the polyacrylamide substrates were determined by performing both unconfined and confined compression testing on cylindrical polyacrylamide specimens using a custom-built compression setup [28].
The adsorption isotherms of cellulosic substrates were determined as follows.
After purification, the catalytic activities towards different substrates were determined.
Kinetic constants for synthetic and natural substrates were determined using Hanes plots (Table 1).
Elemental compositions of substrates were determined with an elemental analyzer (Vario EL cube, Germany).
Characteristics of the substrates were determined beforehand as demonstrated by Figure13 and Figure14.
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