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Reaction rates were measured for both substrates using the same assay.
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For the parental strain the specific activity for both substrates using both co-factors was negatively affected by the increase in temperature (Table 2).
We performed pathway analysis on the predicted Ste20p substrates using the Gene Ontology (GO) [45].
For both types of substrates used, the typical sizes of nanoislands were in the range of 15-30 nm.
The fabrication methodology can be divided into two parts: (1) the growth of CNT bumps on both sides of the substrate using the PECVD approach and (2) the alignment and 'insertion' of the CNT bumps into each other using a flip chip bonder machine.
SASA values were calculated for each potential substrate using the POPS (Parameter OPtimized Surfaces) algorithm.
Sections were developed with diaminobenzidine (Vector Laboratories) substrate using the avidin biotin horseradish peroxidase system (Vector Laboratories).
Therefore, the enzymatic composition of a cocktail can be predicted from any substrate using the models.
The reaction product (brown) was visualized with 3,3′-diaminobenzidine substrate using the Vector ABC Vectastain Elite Kit Vector Laboratoriess, Burlingame, CA).
If you're using a tray filled with substrate, use the syringe to inject the substrate in several places.
Kinase-substrate assays were performed with the 16 protein kinase substrates using both the BSA and BSA-free protocols.
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