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Ethics approval was not required, as both studies were considered to be audit.
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However, the heat shock-induced AeSCP-2 RNAi has similar biological effects on larvae and adults compared to AeSCP-2 dsRNA microinject [17] when comparable stages in both studies are considered.
The data from both studies are considered as one trial for reporting purposes (fig 1).
Although both included studies were considered to have a low risk of bias according to the Cochrane Back Review Group [ 13], both had methodological weakness in blinding of participants and patient compliance, which appear to be common issues in trials of manual therapy interventions [ 25].
Both prospective and retrospective studies were considered.
Both animal and human studies were considered.
Both published and unpublished studies were considered to be eligible for inclusion.
All ACTG clinical studies were considered, including both randomized and non-randomized designs of all phases (I-IV).
Both randomized clinical trials (RCTs) and observational studies were considered.
Both community- and outpatient-based prospective observational studies were considered.
Although extremely widely used and cited, these studies were considered worthless by some geneticists.
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