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Recent additions to the DAS protocol have enabled both structure and alignment (sequence and structural) data to be exchanged.
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D efinition 2 (Sparse Structure and Alignment Space).
Furthermore, the identification of such regions can aid both structure determination and sequence alignment, and may also aid in drug design.
We hypothesize that guide alignments are more accurate because they are the outcome of manual curation that reviews both structure-based alignments and patterns of sequence conservation, where the latter may overrule structure superimposition.
Figure 1 Core structure and dataset alignment.
We don't slight the importance of structure and strategic alignment.
The quality of these alignments was assessed against the implicit sequence alignments generated by the structure-to-structure alignment program TM-align (Zhang and Skolnick, 2005).
Our large-scale experimental test also indicates EPAD is much better than those context-independent distance-based pairwise potentials, such as DOPE Shenn and Sali, 2009), DFIRE (Zhou and Zhou, 2009) and RW (Zhang and Zhang, 2010) in ranking protein alignments (or template-based models) generated by both threading and structure alignment tools (Zhao and Xu, 2012).
Fig. 2 Sequences and structure alignment of native IFN-CSP and Mu-IFN-CSP.
This software was used as a novel genome-wide computational approach to detect miRNA in animals based on both sequence and structure alignment; it has higher sensitivity and comparable specificity than other reported homologue searching methods.
This algorithm, which we call RNAG, iteratively block samples from the conditional probability distributions P(Structure | Alignment) and P(Alignment | Structure), and in so doing refines the models of both Alignment and Structure.
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