During productive NV replication, both structural and nonstructural proteins are detected within cells (5 ).
Full length elements (FLE) are composed of two LTRs that flank gag and pol open reading frames (ORFs), which encode structural and replication proteins, respectively.
Virus entry by membrane fusion releases the viral capsid containing genomic RNA and viral structural and replication proteins into the cytoplasm.
The ViraPower Lentiviral Expression System (Invitrogen, Carlsbad, CA, USA) was used for an optimised mix of packaging plasmids which supplies the structural and replication proteins that were required to produce Lentivirus in 293FT cells.
Preliminary sequencing of essential structural and replication genes, along with random fragments, indicated a considerable level of nucleotide identity between these two phages, in spite of their different geographic origins.
Similar to CP220 and CPt10, CP21 contains four closely related repeat regions (RR1 to RR4, 1 to 2.9 kb in size) which divide the genome into four modules (A to D, 28 to 55 kb, Fig. 2 and Fig. 5a), three of which carrying genes for structural and replication proteins.
CST has both structural and functional homology to the DNA replication protein complex RPA (Miyake et al., 2009); it binds to single-stranded DNA (Miyake et al., 2009) and directly interacts with and stimulates the activity of DNA polymerase α (Casteel et al., 2009).
UvrD appears to be both structural and functional homolog of Srs2 as it also disrupts formation of RecA filaments and has a role in replication fork maintenance [89,90].
The regions of greatest protein conservation occur in the central portion of the genomes, which contain the predicted structural and DNA replication proteins.
