Sentence examples for both strands with an from inspiring English sources
Sequences were recorded in both strands with an overlap of at least 30%.
Each purified PCR product was fully sequenced on both strands with an ABI310 automated DNA sequencer (Perkin-Elmer).
Sequences were obtained on both strands with an ABI 3730xl DNA Analyzer (Life Technologies Corporation, Carlsbad, CA, USA).
Purified DNAs were sequenced on both strands with an ABI 377 DNA sequencer (Davis Sequencing, Davis, CA) and eight pairs of WNV-specific primers.
Twelve sequencing primers were designed on both strands with an average distance of 500 bp, whereas the most outlying primers were designed as nested primers to avoid sequencing background of unspecific PCR products (Table 4).
Amplified DNA fragments were purified with the Qiaquick PCR purification kit (Quiagen, Courtaboeuf, France) and sequenced on both strands with an automated ABI 337 sequencer (Applied Biosystems, Foster City, USA).
This cDNA clone was subcloned and the sequence of human DOR was obtained by sequencing both strands with a two-fold coverage minimum.
PCR products were treated with ExoSAP-IT (USB Corporation Cleveland Ohio, USA), directly sequenced on both strands with a Big Dye Terminator sequencing Kit (v3.1 Applied Biosystem) and run on an Applied Biosystems ABI 3130 XL Genetic Analyzer Applied Biosystemm).
PCR products were treated with ExoSAP-IT (USB Corporation, Cleveland, OH), directly sequenced on both strands with a Big Dye Terminator sequencing Kit (v3.1 Applied Biosystems), and run on an Applied Biosystems ABI 3130 XL Genetic Analyzer Life Technologiess).
PCR products were treated with ExoSAP-IT (USB Corporation, Cleveland, OH), directly sequenced on both strands with a Big Dye Terminator sequencing Kit (v3.1, Life Technologies), and run on an Applied Biosystems ABI 3130 XL Genetic Analyzer Life Technologiess).
