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Both strands were searched for motifs.
The same profile-based search strategy was applied on the FUN, PLN, ENV and INV sections of the release 109 of the EMBL: both strands were searched and 127 577 intervening sequences with a length from 1 to 500 nt were retained as ITS1 to constitute a reference dataset.
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Remaining DMFS parameter settings were: minimum motif length l = 5, maximum motif length m = 10 (with both DNA strands being searched); and at most M = 2 mismatches, when aligning elicited motifs to classification set sequences.
Zinc regulation protein binding motifs, which are AT rich palindromes on the intergenic region of DNA strand were searched using MEME/MAST [ 46].
BioProspector was used with the same motif lengths, only the forward strand was searched, and top 3 scores were reported.
BLAST was accelerated with parameters '-S 1 -e 0 -m 8' so that only the top strand was searched for significant matches and minimal disk writes were required.
We ran MEME on the putative promoter regions of genes in expression clusters with the following parameters: motif width was allowed to range between 8 and 16, 'zoops' and 'tcm' models were both tried for each case, and both strands of the promoter were searched.
One of each 7-mer - reverse complement pair was then removed to avoid double-counting as both strands of upstream sequence were searched for 7-mers.
Motif width was allowed to range between 7 bp and 10 bp, and both strands of the promoters were searched.
Both strands of each sequence were searched and possible motif locations were identified using the motif matrix score obtained from the Gibbs Motif Sampler software.
Both strands of the promoter sequence were searched for the degenerate consensus sequences using the EMBOSS program fuzznuc allowing zero mismatches.
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