Sentence examples for both strands were determined from inspiring English sources

Nucleotide sequences of both strands were determined, directly on PCR products.

To ensure the accuracy of the reactions, nucleotide sequences from both strands were determined.

Nucleotide sequences of both strands were determined with the BigDye terminator cycle method using an ABI3100 Genetic Analyzer (Applied Biosystems).

Sequences for both strands were determined from either PCR products or cloned fragments with custom primers (7 ).

The sequences of both strands were determined using the universal primers (M13RP1 and −21 M13; Applied Biosystems) in Cytb and the primers used for PCR in CR.

To ensure the accuracy of the nucleotide sequences, the sequences on both strands were determined, all polymorphisms were visually confirmed, and for ambiguous polymorphisms PCR amplification and sequencing were repeated.

The nucleotide sequences for both DNA strands were determined.

For all PCR products, sequences from both DNA strands were determined twice.

Sequences from both DNA strands were determined twice for all PCR products.

The platinated siRNAs were also characterized with respect to thermal melting properties, and the number of platinum adducts on the different sense strands were determined by MALDI-ToF MS. In all cases, platination was accompanied by a decrease in melting temperature.

The free energy (ΔG) of RNA duplex formation for the 5 bases at the 5' end of the sense and antisense strands was determined using the thermodynamic parameters and expanded nearest-neighbor model of Xia et al. [ 38].