Sentence examples for both strands using an from inspiring English sources
Each purified PCR product was fully sequenced on both strands, using an ABI310 automated DNA sequencer (Perkin-Elmer).
PCR products were sequenced on both strands using an Applied Biosystems 3730xl DNA Analyzer (Iowa State University DNA Sequencing Facility).
Samples positive for the expected 945 bp band obtained with the SCCA-derived primers were directly sequenced in both strands using an ABI PRISM BigDay terminator ready reaction kit following the manufacturer's instructions (Perkin Elmer Cetus, Emeryville, CA, USA).
PCR products were purified from agarose gels using GELase (Epicenter) and directly sequenced on both strands using an ABI PRISM 3700 DNA Analyzer (Applied Biosystems), and Big Dye (Perkin-Elmer, Applied Biosystems).
Polymerase chain reaction products were purified using ExoSAP-IT (United States Biochemical) and then sequenced on both strands using an ABI 3730 (Applied Biosystems) at the Indiana Molecular Biology Institute.
Double-stranded PCR products were purified directly from the PCR product or from agarose gel using the MinElute purification kit (Qiagen) or Amicon Ultrafree-DNA columns (Millipore) and sequenced directly on both strands using an automatic sequencer CEQ2000 (Beckman) or an ABI 310 (PE Applied Biosystems).
We then searched both strands using a standalone installation of the RED motif prediction algorithm [ 79].
PCR products were purified using the QIaquick PCR purification kit (Qiagen) and directly sequenced on both strands using a Beckman CEQ 8000 automatic sequencer.
The PCR product was sequenced on one or both strands using a BigDye Terminator v3.0 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and run on an ABI 377 automated sequencer.
The nucleotide sequences were determined from both strands using a BigDye Terminator Cycle Sequencing Core Kit (Perkin Elmer, Foster City, CA, USA) and an automated ABI PRISM 377A DNA Sequencer (Applied Biosystems, Foster City, CA, USA).
Each PCR product was directly sequenced on both strands using a dye terminator cycle sequencing kit (Perkin Elmer, Norwalk, CT, USA) and the sequencing products were resolved on an automatic fluorescent DNA sequencer ABI Prism 377 (Applied Biosystems, Valencia, CA, USA).
