Sentence examples for both strands by using from inspiring English sources
PCR products were purified with QiaQuick PCR or gel purification kits (Qiagen, Valencia, CA) and were directly sequenced on both strands by using ABI Prism BigDye terminator kits and an ABI 3130xl sequencer (Foster City, CA).
The sequence of a 360-bp portion of the gnd gene was established on both strands by using primers gnd-1F (TGA AGC AGC AAA CAA AGG TAC) and gnd-8R (TCA TCG GCG ATC TGC TTA AAG T), which amplify an internal portion of 457 bp of the gene.
The purified plasmids were directly sequenced on both strands by using a Rhodamina Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystem) and an ABI PRISM 310 DNA Sequencer (Applied Biosystem), Primer pairs for sequencing were M13 forward (−20) and M13 reverse included in the kit.
Enterovirus VP4 gene amplicons were sequenced on both strands by using the PCR primers.
All sequences were determined for both strands by using the ABI377 automated DNA sequencer (Applied Biosystems).
PCR products were sequenced on both strands by using Genome Express (Meylan, France) and the same primers as for amplification.
Sequence reactions were performed for both DNA strands by using the ABI PRISM Dye Terminator Cycle Sequencing Reaction Kit (Applied Biosystems) in a 20 µl volume with 2 µM of each primer.
Nucleotide sequencing reactions were performed on both amplicon strands by using an ABI PRISM Big Dye Cycle Sequencing Kit with the ABI PRISM 3100 automatic sequencer (Applied Biosystems).
Products were purified by precipitation with 20% polyethylene glycol 2.5 mol/L NaCl and sequenced from both DNA strands by using internal nested primers (Table 2) and BigDye Ready Reaction Mix (ABI, Warrington, UK) according to the manufacturer's instructions.
Tumor samples exhibiting abnormal band migration by SSCP were sequenced on both the forward and reverse DNA strands by using P-labeled cycle sequencing methods.
A method for stress measurement of steel strands by using electromagnetic oscillation (EMO) circuit only at its both ends, on the basis of magnetic-elastic effect, is proposed in this paper.
