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Both strains were fed on bacteria induced to express cuti-1 dsRNA.
Moreover, during lactation, C and LP dams from both strains were fed with control diet.
During gestation, dams of both strains were fed a control diet (C: 192 g protein/kg- 20% of energy) or an isocaloric low protein diet (LP: 81 g protein/kg- 8% of energy) purchased from Hope Farm (Woerden, Netherlands Hary block).
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To increase the acetyl-coA pool, the strains were fed methyl oleate and acetate as carbon sources.
Control strains were fed with HT115 bacteria expressing the empty RNAi vector.
When larvae of the Oregon-R (OR) or other two CS wild-type strains were fed Pf-5 at 107 cfu/plate, none pupariated.
The two strains were fed under the same conditions to 7 weeks of age.
The two mouse strains were fed either a low-fat (regular) diet or a high-fat (Western) diet.
The engineered strains were fed with glycerol as carbon source if they carried the recombinant plasmids using araBAD promoter, otherwise they were fed with glucose.
The two strains were fed under the same conditions (ad libitum feeding, the same food stuff) to 7 weeks of age.
Two different mouse strains were fed with high-or low-fat diets, and compared with control mice and models in which diabetes was induced using streptozotocin (STZ).
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