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Interestingly, the levels of secretory stress appear reduced in the strains containing the murine IgG1 signal peptide and volumetric productivity was significantly increased for both strains compared to those utilising the α-MF signal peptide.
Tonic current amplitude in the same thalamocortical neurons was also reduced in both strains compared to control conditions (WT: 92.8±15.1 pA, SSADH−/−: 167.5±17.9 pA), but not significantly (Fig. 1D).
In the presence of galactose, the xylose consumption rate increased for both strains compared to when xylose was the sole carbon source.
However, the overall level of LPS in wild-type supernatants was not reduced after exposure to phenamil, but, rather, it trends toward being slightly higher for both strains, compared to the DMSO controls.
In the capillary endothelium of the skeletal muscle, there was less dysregulation by HFD in both strains compared to the aortic transcriptional response, suggesting a greater role for galectin-3 in the macrovascular complications of diabetes compared to microvascular disease.
Twenty-four hours following LPS+PGN injection, plasma ASAT and ALAT levels were significantly increased in both strains compared to control, however, no difference between both strains was observed (Fig. 4f,g).
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Reduction in ALP specific activity correlated with reduced amounts of mALP in BBMV from both resistant strains compared to vesicles from susceptible strains as detected in Western blots (Fig. 5C).
Fibrosis increased progressively from day 3 till 14 as reflected by a marked upregulation of collagen in obstructed kidneys of both animal strains compared to the contralateral kidney (Fig. 4).
Although not all parameters were determined in both strains, those analyses performed in parallel indicate that their phenotype is largely identical: Neither of the mutants showed an altered internal period length in DD, and the differences observed in chi square periodogram amplitude as well as in drinking behavior were the same for both mutant strains compared to their respective controls.
In addition, complementation of RHΔ gra2 with either RH-ERP GRA2 or GT1 GRA2 partially restored the p-IκBα localization phenotype, with increased PVM recruitment of p-IκBα present in both complemented strains compared to RHΔ gra2 (Additional file 9: Figure S4A).
Also LV function was equally impaired in both hypercholesterolemic mouse strains compared to age- and diet-matched C57Bl/6J mice.
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