Sentence examples for both steps were combined from inspiring English sources

The supernatants of both steps were combined, dried in a SpeedVac centrifuge, redissolved in 0.8 μl MALDI matrix solution (3.2 mg/ml α-cyanohydroxycinnamic acid (Sigma) in 65% v/v ACN/0.1% v/v TFA), spotted onto 384-well stainless steel MALDI plates and air-dried.

The ethyl acetate supernatants from both extraction steps were combined and evaporated to dryness in a vacuum centrifuge.

The supernatants of both centrifugation steps were combined and frozen in liquid nitrogen until use.

Supernatants from both elution steps were combined (P fraction) and acidified with 5 μl of pure TFA and dried using a SpeedVac.

Pellets from both the first and second centrifugation steps were combined into 4 ml of serum-free DMEM/F12 and 40 μl of DNase (2 units/ml) was added to the cell suspension.

Three pellets resulting from the previous centrifugation steps were combined for DNA extraction.

In this method, the physical disruption and chemical lysis steps were combined, and some parameters in the key steps were adjusted.

The supernatants harvested from these two steps were combined to make the cytoplasmic fraction, and the pellet left was the nuclear fraction.

In the CLU method, the physical disruption and chemical lysis steps were combined, and the key parameters of the incubation temperatures of lysozyme and RNase were adjusted to 60 and 30 °C, respectively.

The peptides extracted in the four steps were combined together, concentrated by a SpeedVac to a desired volume and subjected to LC-MS/MS analysis.

The different weights resulting from the three steps were combined into one overall weight.