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Both steps are carried out at ambient pressure.
Both steps are carried out for a range of possible tracks, intensities, and sizes of the tropical cyclones, to properly characterize the risk in a probabilistic way.
Both these steps were carried out with and without supercritical carbon dioxide.
Both PCR steps were carried out in an Omni PCR UV hood to minimise contamination (Bioquell, Andover, Berkshire, UK).
Both medium exchange steps were carried out with a plate washer ELx50-166, BioTek).
Both sand fly infection steps were carried out following the procedure detailed in the next subsection.
All steps were carried out on both samples, side-by-side, from RNA extraction through sequencing.
All subsequent steps were carried out at 4 °C.
Transient simulations with fine time steps were carried out.
For this purpose, physical models of four steps were carried out on stepped spillways.
All following incubation steps were carried out at room temperature for 1 h.
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