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Clones from both standard and normalized libraries are present on the array.
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Each sample was analyzed in triplicates, and the amounts of templates were estimated by linear regression against the known standard and normalized to internal control.
Concentrations were calculated according to the standard and normalized to the seeded number of cells among different samples.
This work provides guidance on testing and evaluating detection devices, and may contribute to the establishment of suitable standards and normalized procedures.
Absorbance values were compared to standards and normalized by fly weight.
Quantification of BH4, BH2, and B was done by comparison with authentic external standards and normalized to sample protein content.
Quantitation of neopterin was carried out by comparison with external standards and normalized for sample protein content.
Analysis was performed using Bio-Rad CFX Manager software and expression values were based on 10-fold serial dilutions of standards and normalized to Actin levels.
Absolute quantification was done using plasmid DNA constructs encoding respective genes as standards and normalized to express as gene/actin mRNA level.
The absolute amount of transcripts were quantified relative to the standard curve and normalized to actin as a normalization housekeeping gene.
In the second step, the POD is applied to the time-varying means, standard deviations and normalized fluctuation.
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