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For the gonad analysis we chose to treat duplicates individually and only where both spots were significant (P < 0.05) was the spot accepted.
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Forty-two of the 140 protein spots were significant when applying the false discovery rate (FDR) correlation.
The protein spots that were significant (|ratio| > 2.5, P < 0.05) increased or decreased in samples compared with control samples were chosen for further analysis.
Differences in spot volumes were significant if the average ratio of the compared spot volumes was above the calculated value for 2 standard deviations (2SD; Table 1).
Expression changes in 13 (all 19 except spots 1, 120,100,110,121 and 295) spots, regulated after hypoxia, were significant as estimated by the student T-test (< 0.05).
Statistical significance (P < 0.05) was achieved for 222 spots being differentially expressed in inflamed versus non-inflamed mucosa, and of these 39 spots were highly significant (P < 0.0001).
A further 663 grids (22 %) were significant hot spots for at least three types of crime.
Zou et al. optimized the evaluation parameters and standards of the tight oil "sweet spots" which are significant for the exploration of tight oil in China.
Some spots could be significant in multiple comparisons.
The spots that are significant when using one-way ANOVA and FDR correlation could be considered proteins that are significant as biomarkers, although the biological significance of these proteins must be considered.
A quintessentially English, poorly educated, working-class war veteran with social and intellectual aspirations, whose fictional address 23 Railway Cuttings, East Cheam perfectly conjures the aspirant bleakness of London's suburbs (as if Cheam were significant enough a spot to have an East).
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