Data from multiple sources were pooled or meta-analysed.
For comparisons between GBM (JHGBM-276, JHGBM-551, JHGBM-854, and JHGBM-965) and fetal cells (F34, F48, and F54), results from all cell sources were pooled to form a single cancer group and a single noncancer group.
A variety of pseudogene annotations from multiple sources were pooled and filtered to obtain a subset of sequences that have significant mid-sequence disablements (frameshifts and premature stop codons), and that have clear evidence of full-length mRNA transcription.
Using a new computational approach, a variety of Alu-related sequences from multiple sources were pooled and filtered to obtain a subset containing Alu elements and characterized miRNA genes for which there is clear evidence of full-length transcription (embedded in EST).
An advantage of this approach is its consistency with consumer demand models which assume that income from diverse sources is pooled.
Equal amounts of total RNA from each source were pooled for Illumina mRNA-Seq.
Equal amounts (4 μg) of RNA from each source were pooled, and poly (A)+ RNA was isolated, reverse transcribed into cDNA and paired-end sequenced (Illumina, 2 × 101 bp) (SciLifeLab, Stockholm).
In addition, for some tests we had a larger dataset to analyze because data from both sources were able to be pooled for analysis.
However, no correlations were found when soils from all the sources tested were pooled [134].
Transcript annotations from multiple sources (Methods) were pooled and extended using EST-indicated polyadenylation sites drawn from PACdb [26] (Figure 1) to produce 57,875 distinct transcripts.
