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Both solutions were pooled together and evaporated in vacuo to afford 50 g of crude extract.
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Effluent solutions were pooled into separate aliquots every 40 min for analysis.
After 5 min, the solutions were pooled.
The two bacterial solutions were pooled to make a total volume of 150 µl.
The two spore solutions were pooled to make a total volume of 145 µl.
The extracted solutions were pooled and evaporated to dryness under vacuum.
The extracted solutions were pooled and evaporated to dryness in a SpeedVac vacuum centrifuge.
The two bacterial solutions were pooled to make a total of 350 µl.
After 5 min, the solutions were pooled and incubated for 10 min. The culture medium was then replaced by the DNA-Transfast solution.
Lipids (in chloroform solutions) were pooled in cryovials (a total of 13.5 µmole in each cryovial) and dried under vacuum until all of the solvent was removed.
After 30 minutes, the two solutions were pooled and added to the culture medium 10 min later in a final volume of 1 mL.
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