Sentence examples for both sets of primers from inspiring English sources

Both sets of primers showed satisfactory results.

Species-specificity of both sets of primers was evaluated by using 27 reference strains of the DSM collection, representing 23 different species of the Staphylococcus genus and 3 species of the Kocuria genus.

Both sets of primers amplify 132 bp, including 84 bp of the mature conotoxin coding region and 48 bp of the 3' untranslated region.

Amplification reactions for both sets of primers were made in a volume of 50 µl containing 1.25 U of Ex Taq Polymerase (Takara), 1× reaction buffer, 2 mM MgCl2, 20 pmol each primer (IDT), 200 µM dNTP and 100 500 ng of template DNA.

In total 199 DNA samples from 182 species were analysed with both sets of primers.

Results that were positive for both sets of primers were considered positive.

All individuals were PCR-amplified and sequenced using both sets of primer pairs.

The second round of screening with both sets of primer pairs was performed as described above except that an annealing temperature of 56ºC was used.

Twenty-four (35%) of the samples were positive by both set of primers and therefore were considered positive.

Munoz et al. used both sets of degenerate primers in a multi-centre case-control study detailing the risks of cervical cancer associated with different HPV subtypes and found a HPV detection rate of 90.7% [ 5].

To investigate the possible infection of non-canine mammalian species, we screened available plasma/serum, respiratory, and liver samples from horses (n = 175), donkeys (n = 16), domestic cats (n = 56), pigs (n = 40), and wild mice (n = 61) by using both sets of conserved primers (Table 3).