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We simulated data using our best fitting parameters and compared the results with XCI in both sets of cell lines (Figure 4).
Furthermore, we made a point of manipulating the PI3K pathway in both cell lines with high PI3K scores and cell lines with low scores, and both sets of cell lines appear to yield similar results.
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Then they used optogenetics to make both sets of cells fire together.
Finally, for both sets of cells, the directional accuracy was best when the overall concentration of α-factor was closest to the receptor Kd, indicating the average level of α-factor exposure was an important determinant of accurate gradient-sensing.
For hybridomas, details of the sources of both sets of cells are needed.
Both sets of cells were used for experiments within 2 to 3 days after plating.
Drug treatment conferred a significant colony formation advantage in both sets of cells, which exhibit equivalent paradoxical ERK activation.
Both sets of cells were expanded into 175 cm flasks, treated with sodium butyrate and left for three weeks.
This procedure assured similar cell densities for both sets of cells at the time of photosensitisation, which was confirmed by actual cell counts in parallel dishes.
Work performed in mouse embryos has hinted that both sets of cells develop from cells that produce a protein called Mesp1.
Since the data from both sets of cells were derived under similar conditions and with the same algorithmic tools, they would presumably have similar rates of false positives and negatives.
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