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This suggests that when both sequencing and mutation analysis are 100% successful, 8% of patients (57 minus 49) would receive anti-EGFR therapy in vain when only exon 2 of KRAS would have been assayed.
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These samples were negative for EGFR mutations by both sequencing and HRM.
Both the repeat sequence and mutation information were encoded and processed by an efficient graph kernel to learn higher-order correlations.
An alteration frequency score for each gene was calculated, based on the 84 GBM cases with both sequence mutation and copy number data (hypermutators excluded).
Both sequence mutation and alteration in methylation pattern were detected.
Helfer, E. et al. Endosomal recruitment of the WASH complex: active sequences and mutations impairing interaction with the retromer.
The black frames indicate the target sequences; and the PAM sequences and mutations are in blue and red, respectively.
3' UTR sequences and mutations were verified by sequencing.
In principle sequencing and the mutation assays are similar techniques.
Both the frequency of mutated sequences and the mutation rate for splenic B-1a samples from 2 month old mice are substantially lower than those in 4 6 month old mice, further supporting that the SHM in the splenic B-1a IgVH is an accumulative process.
Successful introduction of artificial mutations was confirmed via both Sanger sequencing and the amplification refractory mutation system.
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