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As for the unmodified controls, Kf-exo– binds tightly in both sequences in the presence of the correct dCTP.
Both sequences in the alignment were then trimmed to the extent of the human ORF based on annotation in the RefSeq or GenBank entry.
Consistently, concomitant mutation of both sequences in the 5mut1,2 construct disabled the response of the HCV reporter RNA translation to miR-122 sequestration.
Interestingly, in both sequences in the combination there is a reduction in pHH3 compared with the docetaxel alone group at that timepoint (PD2 and PD7) (3.8- and 2.5-fold change, respectively; P<0.0005).
Only two nucleotide polymorphisms, at positions 60 and 112 (a G-A transition, and a C-A transversion, respectively), characterized both sequences in the two classes from the three species.
Southern blot hybridization of extrachromosomal DNA from various S. citri strains and spiroplasma species to an arp-specific probe and a probe made from the entire plasmid DNA of BR3-3X revealimitedited conservation of both sequences in the genus Spiroplasma.
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To detect both RNA sequences in the same cell, the cre -specific and KAP104 -specific probes were differentially labeled.
Both sequences lie in the respective gene promoter (UCSC Genome Browser assembly GRCh38/hg38).
This is a common phenomenon to highly structured RNA, both with specific sequences in the 5' untranslated region as well as in the 3' untranslated region [63].
Therefore, we included both RNA and DNA sequences in the analysis.
In cases of duplications, both of the duplicated sequences in the Hominini are considered orthologous to single-copy regions in the outgroups.
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