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Integration of gene and miRNA profiling was performed by both sequence complementarity and expression correlation.
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TargetScan is a widely used prediction algorithm that takes into consideration both sequence complementarity (especially the seed regions of miRNAs) and conservativity of binding sites.
The target cleavage requires extensive sequence complementarity and dramatically accelerates core-RISC recycling.
The Cas9 proteins are type II CRISPR-associated, RNA-guided DNA endonucleases that identify double-stranded DNA targets by sequence complementarity and protospacer adjacent motif (PAM) recognition.
The minimum sequence complementarity and thermodynamics of hybridization required for a region of mRNA to serve as a miRNA target site in vitro are not fully understood.
We created miRNA association network by using miRNAs sharing target genes based on sequence complementarity and co-expression patterns of miRNA-target pairs.
They bind to the 3′-untranslated region (3′UTR) of target mRNA based on sequence complementarity and result in target mRNA degradation or suppression of translation [3], [4].
The prediction was performed with the default parameters such as the degree of miRNA:target sequence complementarity and the free energy level of RNA-RNA duplexes.
In many cases the sRNA:mRNA interaction occurs over short regions of imperfect sequence complementarity and thus requires stabilization by the RNA chaperone Hfq [ 7].
Roles of sRNAs were predicted by sequence complementarity and our results showed that many sRNAs identified in this work might be directed against various transcription factors.
In animals, miRNA target sites most often show partial sequence complementarity and, although best characterised in 3'UTRs, can occur anywhere in the gene [ 3, 4].
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