This predictive ability is most likely due to the algorithms within the dotplot that allow for alignment of sequences with a high mosaic nature, both in sequence and orientation.
Sequence and orientation of the CMV promoter and EGFP gene were confirmed by DNA sequencing.
The construct was sequenced to ensure that the sequence and orientation were correct.
Sequence and orientation of a neomycin-resistance gene was confirmed by DNA sequencing.
The sequence and orientation of the DNA construct was verified by DNA sequencing.
Clones were tested for correct insert sequence and orientation using the M13 forward and reverse primers.
Sequence and orientation of cloned vTK was confirmed by sequencing (University of Alberta, DNA Sequencing Core Facility).
Sequence and orientation of pDEST and the R1-ccdB-CmR-R2 cassette was confirmed by DNA sequencing.
The correct sequence and orientation was verified by sequencing.
The correct cDNA sequence and orientation were confirmed by sequencing the pGYT-HA-RASSF1C plasmid.
