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This would imply that both sense and antisense transcript would be equally amplified.
Thus, both sense and antisense transcript levels are regulated during the IDC but regulation is not synchronous.
Additionally, both sense and antisense transcript abundances were estimated by binning reads according to their start position against the TAIR9 genome annotation.
At all three loci, both sense and antisense transcripts can be detected, albeit with antisense transcripts at much lower level than the sense transcript.
Examination of enriched replicative histone transcripts in tdp-1 mutants showed that both sense and antisense transcripts originating from histone genes were observed in tdp-1 ok803 tdp-1 ok803ype totandRNA-seq data.
Strand-specific RT-PCRs detected transcripts in both directions for these loci (albeit at a lower level for the antisense transcript), implying that RdRP-based small RNA generation could occur for both sense and antisense transcripts in this parasite.
Strand specific RT-PCR showed both sense and antisense transcripts can be detected for group II gene loci, suggesting that bi-directional transcripts are available to serve as templates for small RNA generation.
This suggests even though both sense and antisense transcripts are present at the same time, there is a net abundance of sense transcripts.
Detectable expression of both sense and antisense transcripts containing the hexanucleotide repeat in C9ORF72 patients indicates that bidirectional transcription also occurs in c9FTD/ALS [ 25].
Interestingly, both sense and antisense transcripts of both elements are expressed in the same cells.
Another difference is that, in the case of F elements, both sense and antisense transcripts are mainly polyadenylated.
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