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Both samples were vortexed (1 min), centrifuged (3,000 g, 3 min), and counted for radioactivity.
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Both samples were vortex-mixed and incubated at room temperature for 30 s.
The samples were vortexed adequately before pipetting.
All the samples were vortexed for 1 min to give blank samples.
Samples were vortexed for 1 min, and centrifuged at 10,000 rpm for 10 min at 4 °C.
These samples were vortexed and centrifuged at 12,000×g for 20 min at 4 °C.
Then the samples were vortexed for 30 s and centrifuged at 4000 rpm for 10 min.
To this sample, 500 μL of 100 mM ammonium acetate buffer was added and then samples were vortexed.
A volume of 600 μl of CHCl3 was then added and the samples were vortexed for 30 s.
Samples were vortexed again and allowed to react at RT for 1 h.
Samples were vortexed for 10 sec and centrifuged for 30 sec (4°C, 13 200 rpm).
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