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In the next step, both samples were reduced with dithiotreitol and alkylated with iodoacetamide, followed by trypsin digestion at 37 °C overnight.
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In our example, where we analysed only core probesets, 136 233 probesets of 284 258 were deemed present by our filter, and the number of transcript clusters to analyse (present in both samples) was reduced from 18 708 to 8401.
Both Pd technologies stored more NO when the samples were reduced, indicating that metallic Pd is effective for storing NO.
Protein samples were reduced, alkylated and digested with trypsin.
Secondary silica samples were reduced to millimeter-size (~ 2 4 mm) silica chips.
Briefly, NO reaction products in plasma samples were reduced by vanadium chloride.
The samples were reduced with H2 without or after calcination in air or helium.
Negative matrix effects due to the complexity of the studied samples were reduced by dilution with ultrapure water.
For the isotopic analysis, further 100 mg from selected samples were reduced in powder in an agate mortar.
Next the samples were reduced at 723 K under 5% H2/He flow (50 mL min−1) for 1 h.
The samples were reduced to 10-μm thick using ion milling process to reveal the microstructure under TEM.
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