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Socioeconomic status (SES) and ethnic background in both samples were assessed by identical parent questionnaires.
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Each experiment was run twice with a least 6 animals per group, and in both cases, samples were assessed in triplicate.
Each reaction was run twice with a least 6 animals per group, and in both cases, samples were assessed in triplicate.
Both these samples were assessed with InterRAI instruments.
After both thawing and rehydration, samples were assessed for viability by Syto-13/PI staining, for CD34+-presenting cells by fluorescence-activated cell sorting (FACS) and for colony-forming units (CFU) by assay.
The integrity and purity of all the samples were assessed both on 1.2% formaldehyde agarose gel and UV Spectrophotometer at A260: A260.
Genetic diversity and structure of barley samples were assessed through both population-based and individual-based analysis.
Total RNA extraction was performed with Reliaprep RNA tissue miniprep system (Promega) following manufacturer indications, and both quality and integrity of the RNA samples were assessed with the Agilent 2100 Bioanalyzer (Agilent Technologies).
The filtered reads from all 136 samples were assessed by MultiQC.
The concentration and integrity of all RNA samples were assessed using the NanoDrop ND-2000 spectrophotometer (NanoDrop Technologies).
Blood samples were assessed before and after official marcha contest.
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