For the extraction of total RNA from both FFPE and FF breast tissue samples, we used the High Pure RNA Paraffin Kit Roche Applied Sciencee, cat# 03 2703289 031).
For both CD34+ and stromal cell samples we used the following parameters: two-class unpaired responses, t-statistic, 500 permutations.
In order to overcome the challenges of collecting sufficient numbers of tissue samples, we used both commercial and institutional resources.
For both types of sampling we used gill nets (3 m high by 91 m long) with mesh sizes measuring 5-, 10-, and 15-centimeters (stretch mesh).
For sampling, we used both purposive and snowballing approaches (Green and Thorogood 2004).
Although we analysed a German patient sample, we used both EQ-5D indexes (being aware of the limited comparability between both populations).
To avoid any protein losses from the sample, we used both supernatants and precipitates of these fractions for 2-DE analysis.
Despite the analysis being of a German patient sample, we used both EQ-5D indexes because the estimation of the EQ-5D index-G was based on a rather small sample (nGerman = 334 vs. nUK = 2997) and on the valuation of fewer health states (36 vs. 43).
For both samples, we then used the QIAamp viral RNA Mini Kit to isolate viral RNA from cell culture supernatants, resulting in 30 µl of RNA with the concentration ranging between 1 5 ng/µl.
It is important because the sample we use is very representative of our target audience.
