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Total RNAs were isolated from both samples using an RNeasy mini kit (Qiagen) after 10-hour infection.
FC data was acquired for both samples using an LSR II cytometer (BD Immunocytometry Systems).
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We also calculate the carrier tunneling time for both samples using a modified form of the semiclassical Wentzel Kramers Brillouin (WKB) approximation [26 28].
To measure the proliferative index of IDPs from both strains we stained samples using an anti-bromodeoxyuridine antibody as shown in Figures 3g 3g 3g.
Total RNA was extracted from both control and cold-treated tissue samples using an RNeasy Mini Kit (Qiagen, Valencia, CA, USA).
In addition, motor and functional recovery patterns were compared between both patient samples, using a generalized linear mixed methods model (GLIMMIX).
Singh, IIT Hyderabad for his help in mechanical characterization of the samples using an Instron microtester.
Hierarchical clustering was performed on both probes and samples using a Pearson centered similarity measure.
Next, four paths, parallel to each other, were melted on both sides of the samples using a laser beam.
XTEM was carried out on both the 900°C PDA samples using a JEOL 2000FX operated at 200 kV.
DNA was extracted from both leaf and cambium samples using a modified cetyltrimethylammonium bromide (CTAB) method (Cullings, 1992).
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